bs 1448r  (Bioss)

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 interacted with Wnt2 and β-catenin in POF mice. ( A ) qPCR was used to detect Wnt-2 and β-catenin mRNA expressions in the ovarian tissues. ( B ) Immunohistochemistry was applied to measure Notch2 protein expression in the ovarian tissues. Magnification×200, scale bar = 100 μm. ( C ) Western blot analysis was utilized to test Notch2 pathway-related protein expressions (Notch2, Jag1 and Hes2) and Wnt2/β-catenin pathway-related protein expressions (Wnt2, β-catenin, Axin2 and LEF1) in the ovaries. ( D ) Co-immunoprecipitation (Co-IP) was conducted to further verify the protein-protein interactions between Notch2 and Wnt2. P < 0.05 and P < 0.01 vs. Control; # P < 0.05 and ## P < 0.01 vs. oe-NC; @ P < 0.05 and @@ P < 0.01 vs. oe-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Immunohistochemistry, Expressing, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein-Protein interactions, Control

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 acted on the Wnt2/β-catenin pathway in POF cells. ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. P < 0.05 and P < 0.01 vs. Control; * P < 0.05 and ** P < 0.01 vs. Model; # P < 0.05 and ## P < 0.01 vs. sh-NC; @ P < 0.05 and @@ P < 0.01 vs. sh-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Western Blot, Control

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: βcatenin knockdown inhibited Wnt2/β-catenin pathway in POF cells treated with sh-Notch2 and SKL2001 ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. # P < 0.05 and ## P < 0.01 vs. sh-Notch2 + SKL2001 + sh-NC. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Knockdown, Western Blot

Journal: Neural Regeneration Research

Article Title: Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity

doi: 10.4103/1673-5374.369124

Figure Lengend Snippet: Effects of HI on Notch1 and Jagged1 expression and Notch1/Jagged1 double-positive immunofluorescence in the hippocampal region in pigs after HI. (A) Notch1/Jagged1 double immunofluorescence staining in the brain of the control group and of the HI groups 0–6, 6–12, 12–24, and 24–48 hours after injury (red fluorescence represents Notch1, green fluorescence represents Jagged1, and blue fluorescence represents nuclei) (scale bar: 20 μm). Images are representative of three other images. (B) Change in mean optical density of Notch1 protein expression. In the 0–6 hours post-HI group, Notch1 expression was decreased, whereas it was increased in the 12–24 hours post-HI group. (C) Change in mean optical density of Jagged1 protein expression. In the 12–24 hours post-HI group, Notch1 expression was increased compared with the 6–12 hours group, whereas it was decreased in the 24–48 hours post-HI group. (D) Change in the percentage of Notch1/Jagged1 double-positive cells. The percentage of Notch1/Jagged1 double-positive cells was decreased at 0–6 hours, increased at 12–24 hours, and decreased again at 24–48 hours after HI. Data are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test for B and C; one-way analysis of variance followed by Dunnett’s t -test for D). All experiments were repeated three times. HI: Hypoxic ischemia.

Article Snippet: The primary antibodies used for immunofluorescence staining and their dilutions were as follows: mouse monoclonal anti-Notch1 antibody (1:50, Abcam, Cat# ab44986, RRID: AB_776840); rabbit polyclonal anti-Jagged1 antibody (1:50, Abcam, Cat# ab7771, RRID: AB_2280547); rabbit polyclonal anti-EAAT2 antibody (1:50, Abcam, Cat# ab203130, RRID: AB_2924794); and rabbit polyclonal anti-synaptophysin antibody (1:100, Abcam, Cat# ab14692, RRID: AB_301417).

Techniques: Expressing, Immunofluorescence, Double Immunofluorescence Staining, Control, Fluorescence